In vitro DIRECT SHOOT REGENERATION FROM Rhodiola rosea L. LEAF EXPLANTS

Wild plant species are of great interest as a source pharmacologically valuable compounds but number them endemic and/or endangered ones. Modern biotechnology can provide reliable methods for their utilization without disturbing natural populations. In vitro culture Rhodiola being intensively developed to include into various biotechnological programmes. Aim. Development protocol direct rosea L. regeneration from leaf explants. Methods. The leaves R. aseptically growing plants were used the Several variants Murashige and Skoog (1962) agar-solidified medium supplemented with different combinations auxins (1-naphthaleneacetic acid (NAA) 2,4-dichlorophenoxyacetic (2,4-D)) cytokinins (kinetin 6-benzylaminopurine (BAP)) estimated potential regeneration-inducing media. Regeneration frequency was calculated percentage that produced shoots. Results. use MS 2.5 mg/l BAP 1.0 2,4-D allowed inducing shoot formation 100% frequency. An increase in content up decrease resulted decreasing 62.5%. 25% 62%, respectively, on media containing kinetin + 2,4-D. Conclusions. explants have demonstrated high capacity using studied growth regulators. 100%. lower case substitution kinetin. other types morphogenesis (formation adventitious roots callus) also observed.